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KMID : 0545120170270050939
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 5 p.939 ~ p.942
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Macromolecular and Elemental Composition Analyses of Leuconostoc mesenteroides ATCC 8293 Cultured in a Chemostat
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Bang Jeong-Su
Li Ling
Seong Hyun-Bin
Kwon Ye-Won
Jeong Eun-Ji
Lee Dong-Yup
Han Nam-Soo
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Abstract
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The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat. The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA. Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine. Elements were in the order of C > O > N > H > S. The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM). These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
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KEYWORD
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Leuconostoc mesenteroides, composition, elements, macromolecules, metabolites
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